Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (C-Term) antibody

Details for Product No. ABIN968455
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Antigen
Synonyms D12S1644, IL-4-STAT, STAT6B, STAT6C
Epitope
C-Term
(51), (36), (22), (12), (10), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(254), (98), (91), (15), (12), (8), (4)
Host
Mouse
(218), (49), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(202), (92), (74), (56), (38), (30), (24), (15), (15), (14), (6), (4), (4), (1)
Pubmed 9 references available
Quantity 50 μg
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Catalog No. ABIN968455
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Immunogen Human Stat6 Recombinant Protein
Clone 23
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Stat6
Background STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta.
The 23/Stat6 monoclonal antibody recognizes Stat6, regardless of phosphorylation status.
Molecular Weight 100 kDa
Research Area Signaling, Receptors, Inflammation
Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (C-Term) antibody Western blot analysis of Stat6 on jurkat lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Stat6.
anti-Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (C-Term) antibody (2) HeLa
Product cited in: Quelle, Shimoda, Thierfelder et al.: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).

Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, 1996 (PubMed).

Mikita, Campbell, Wu et al.: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Dent, Hu-Li, Paul et al.: "T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13823-8, 1998 (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000 (PubMed).

Bartoli, Gu, Tsai et al.: "Vascular endothelial growth factor activates STAT proteins in aortic endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 43, pp. 33189-92, 2000 (PubMed).

Waite, Floyd, Arbour-Reily et al.: "Interferon-gamma-induced regulation of peroxisome proliferator-activated receptor gamma and STATs in adipocytes." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7062-8, 2001 (PubMed).

Xia, Salzler, Kunz et al.: "A novel serine-dependent proteolytic activity is responsible for truncated signal transducer and activator of transcription proteins in acute myeloid leukemia blasts." in: Cancer research, Vol. 61, Issue 4, pp. 1747-53, 2001 (PubMed).

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