CAMK2A antibody (AA 448-460)

Catalog No. ABIN968457

This anti-CAMK2A antibody is a Mouse Monoclonal antibody detecting CAMK2A in WB, IHC, IF and BI. Suitable for Human, Mouse, Rat and Dog. This Primary Antibody has been cited in 5+ publications.

Quick Overview for CAMK2A antibody (AA 448-460) (ABIN968457)

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAMK2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), BioImaging (BI)

Clone: 45-CaM Kinase II

Product Details anti-CAMK2A Antibody

Binding Specificity: AA 448-460

Cross-Reactivity: Mouse (Murine), Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat CaM Kinase IIalpha aa. 448-460

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CAMK2A antibody (ABIN968457)

Fallon, Moreau, Croft, Labib, Gu, Fon: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, (2002) (PubMed).

Zong, Ren, Young, Pypaert, Mu, Birnbaum, Shulman: "AMP kinase is required for mitochondrial biogenesis in skeletal muscle in response to chronic energy deprivation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 25, pp. 15983-7, (2002) (PubMed).

Brocke, Chiang, Wagner, Schulman: "Functional implications of the subunit composition of neuronal CaM kinase II." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22713-22, (1999) (PubMed).

Ishida, Fujisawa: "Stabilization of calmodulin-dependent protein kinase II through the autoinhibitory domain." in: The Journal of biological chemistry, Vol. 270, Issue 5, pp. 2163-70, (1995) (PubMed).

Hanson, Schulman: "Neuronal Ca2+/calmodulin-dependent protein kinases." in: Annual review of biochemistry, Vol. 61, pp. 559-601, (1992) (PubMed).

Target Details for CAMK2A

Target: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Alternative Name: CaM Kinase II

Background: Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) is a multifunctional Ser/Thr kinase that regulates a number of cellular functions in response to increased intracellular Ca2+. CaM kinase II is widely distributed, but is predominantly expressed in brain. It is involved in the regulation of neuronal functions such as neurotransmitter synthesis, neurotransmitter release, long-term potentiation, and formation of spatial learning. Neuronal CaM kinase II contains heteromers of two major subunits, alpha and beta, at a ratio of 2:1 and homomers of alpha subunits. Each subunit has N-terminal ATP-binding and catalytic/regulatory domains and a C-terminal association domain. The regulatory domain consists of the autoinhibitory and calmodulin-binding sites. Assembly of the association domains of multiple subunits positions the regulatory domains for intersubunit autophosphorylation. After binding Ca2+/calmodulin, CaM kinase II undergoes rapid autophosphorylation of the alpha and beta subunits, which results in a substantial increase in its affinity for Ca2+/calmodulin.
Synonyms: Ca2+/calmodulin-dependent protein kinase II

Molecular Weight: 52 kDa

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction