Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT) (AA 681-803) antibody

Details for Product No. ABIN968484
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms NPAT-like, im:6901964, DKFZp469B186, 6820401K01, AI427561, BB112559, E14, E14/NPAT, p220
Epitope
AA 681-803
(1), (1)
Reactivity
Human
(6)
Host
Mouse
(4), (2)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
(2), (2), (2), (2), (2)
Pubmed 1 reference available
Quantity 50 µg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968484
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human NPAT
Clone MK-1-410
Isotype IgG2b
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name NPAT
Background Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.
Synonyms: Nuclear Protein Ataxia Telangiectasia
Molecular Weight 210 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT) (AA 681-803) antibody Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
anti-Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT) (AA 681-803) antibody (2) Western blot analysis of NPAT on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:100, lane 2: 1:200, lane 3: 1:400 dilution of the mouse anti-human NPAT antibody.
anti-Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT) (AA 681-803) antibody (3) anti-Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT) (AA 681-803) antibody (Image 3)
Product cited in: Zhao, Dynlacht, Imai et al.: "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry." in: Genes & development, Vol. 12, Issue 4, pp. 456-61, 1998 (PubMed).

Validation Images
Did you look for something else?
back to top