Immunofluorescent staining of SH-SY5Y cells (right). Cells were seeded in a collagen coated 384 well imaging plate at ~ 8,000 cells per well. After overnight incubation, cells were stained using the Triton X100 fix/perm protocol and the anti-Ataxin antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen). The image was taken on a Pathway 855 or 435 imager using a 20x objective. This antibody also stained SK-N-SH, C6, U87 and U373 cells using both the Triton X100 and methanol fix/perm protocols.