GIT1 (AA 664-770) antibody

Antigen: GIT1

Binding Site: AA 664-770

Clonality: Monoclonal (13)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968517

Additional Information

Immunogen: Rat GIT1

Cross-Reactivity: Mouse (Murine), Human

Format: Liquid

Isotype: IgG2a

Clone: 13

Description: Activation of adrenergic receptors (AR), such as beta2AR leads to activation of heterotrimeric G proteins and recruitment of G protein-coupled receptor kinases (GRKs). Phosphorylation of the activated receptor by GRKs promotes the binding of arrestin protein which prevents further G protein activation. GRK interactor 1 (GIT1) was identified in a yeast two-hybrid screen for proteins that bind GRK2. GIT1 has an N-terminal Zn2+ finger-like motif involved in GTPase activating activity and multipe ankyrin repeat units. In vitro, GIT1 is a GTPase-activating protein (GAP) for the ADP ribosylation factor (ARF) family of small GTP-binding proteins. In rat, GIT1 is widely expressed with the highest levels in the testis and lowest levels in the liver and spleen. Overexpression of GIT1 in HEK293 cells causes reduced beta2AR signaling and increased receptor phosphorylation as a result of reduced receptor internalization and resensitization. In addition, GIT1 interacts directly with paxillin and the PIX exchange factors, and indirectly with PAKs, Rac1, and Nck. GIT proteins are also now known to be PIP3-stimulated GAPs for ARF6. Thus, GIT1 activation of ARF proteins may involve cell signaling pathways that are important for endocytosis, cell adhesion, and cytoskeletal dynamics.
Synonyms: G protein-coupled receptor kinase interactor 1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 95 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of GIT1 on a rat cerebrum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1: 1000 dilution of the mouse anti-GIT1 antibody.

Immunofluorescence staining of A431cells (Human epithelial carcinoma, ATCC CRL-1555) with the mouse anti-GIT1 antibody.

Publications

Product: Premont, Claing, Vitale et al.: "beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 24, pp. 14082-7, 1998 (PubMed).

Zhao, Manser, Loo et al.: "Coupling of PAK-interacting exchange factor PIX to GIT1 promotes focal complex disassembly." in: Molecular and cellular biology, Vol. 20, Issue 17, pp. 6354-63, 2000 (PubMed).