LIM Domain Kinase 1 (LIMK1) (AA 232-333) antibody
|Synonyms||LIMK, limk1, xlimk1, LIMK1, MGC146234, GB16654, DKFZp459P0831|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|4 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Rat (Rattus), Mouse (Murine)|
|Description||Two LIM motif-containing protein kinases (LIMK) have been identified, LIMK1 and LIMK2. These kinases contain two N-terminal LIM domains, a central PDZ domain, and a C-terminal Ser/Thr kinase domain. LIMK1 is highly expressed in brain, heart, and skeletal muscle, while LIMK2 exhibits the highest expression in placenta, liver, lung, kidney, and pancreas. LIMK1 is localized to the actin cytoskeleton and phosphorylates the actin binding/depolymerizing factor, cofilin. During Rho-induced neurite retraction, activation of ROCK leads to LIMK1 activation via phosphorylation at Thr508. In COS-7 cells, disruption of the second LIM domain or the PDZ domain increases LIMK1-induced aggregation of the actin cytoskeleton. In addition, a 32 kDa splice variant that contains only the N-terminus (dLIMK1) suppresses LIMK1 activity by interaction with the C- terminal kinase domain. In humans, deletion of LIMK1 has been implicated in Williams syndrome, a disorder that produces a distinct cognitive profile and vascular disease. Thus, LIMK1, and its splice variant dLIMK1, are thought to have important roles in the regulation of the actin cytoskeleton in a wide varitey of tissues.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||72 kDa|
Related Products: ABIN968546, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments|
|Restrictions||For Research Use only|
|Western blot analysis of LIMK1 on rat cerebellum lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-LIMK1. Immunofluorescent staining of NIH-3T3 cells.|
Okano, Hiraoka, Otera et al.: "Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs." in: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31321-30, 1996 (PubMed).
Frangiskakis, Ewart, Morris et al.: "LIM-kinase1 hemizygosity implicated in impaired visuospatial constructive cognition." in: Cell, Vol. 86, Issue 1, pp. 59-69, 1996 (PubMed).
Edwards, Gill: "Structural features of LIM kinase that control effects on the actin cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 11352-61, 1999 (PubMed).
Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).
|Hosts||Rabbit (63), Mouse (18)|
|Reactivities||Human (79), Rat (Rattus) (38), Mouse (Murine) (35), Cat (Feline) (1), Chicken (1), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1), Monkey (1)|
|Applications||Western Blotting (WB) (62), ELISA (33), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Flow Cytometry (FACS) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||pThr508 (15), C-Term (4), N-Term (4), AA 630-647 (3), AA 548-648 (1), Internal Region (1), Thr508 (1), pTyr507, pThr508, pTyr504, pThr505 (1)|