Exportin 1, CRM1 Homolog (Yeast) (XPO1) (AA 2-122) antibody
|Synonyms||Xpo1, 18.m06600, XPO1, LOC100220104|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|6 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)|
The nuclear pore complex (NPC) is a 125 MDa nuclear envelope complex that mediates nucleocytoplasmic shuttling of RNA and protein. NPC is comprised of more than 30 different proteins, including Ran/TC4, importins, and exportins . Exportin-1(hCRM1/XPO1) is the widely expressed human homologue of the yeast CRM1 protein, which functions in the maintenance of chromatin structure and nuclear export. Exportin-1 has an N-terminal CRIME domain that is homologous to yeast CRM1 and importin-beta. Intracellular localization of Exportin-1 shows association with the NPC and overexpression of Exportin-1 in Xenopus oocytes stimulates Rev and U snRNA export from the nucleus. Exportin-1 also binds other nuclear transport related proteins, such as RanGTP, snurportin-1, and the nucleoporins CAN/Nup214 and Nup50. Overexpression of CAN leads to sequestration of Exportin-1 in the nucleoplasm, which inhibits cell growth and induces apoptosis. In addition, Exportin-1 may export specific proteins, such as Rev and PKI, via binding to a leucine-rich nuclear export signal (NES). Thus, Exportin-1 is an important protein and RNA nuclear export receptor, which may be required for normal cell growth and survival. This antibody is routinely tested by western blot analysis.
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||112 kDa|
Related Products: ABIN968553, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of Exportin-1 on WI-38 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of anti-Exportin-1 antibody. Immunofluorescent staining on SK-BR3 cells.|
Fornerod, Ohno, Yoshida et al.: "CRM1 is an export receptor for leucine-rich nuclear export signals." in: Cell, Vol. 90, Issue 6, pp. 1051-60, 1997 (PubMed).
Boer, Bonten-Surtel, Grosveld: "Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects, and apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1236-47, 1998 (PubMed).
Paraskeva, Izaurralde, Bischoff et al.: "CRM1-mediated recycling of snurportin 1 to the cytoplasm." in: The Journal of cell biology, Vol. 145, Issue 2, pp. 255-64, 1999 (PubMed).
Ishida, Hara, Kamura et al.: "Phosphorylation of p27Kip1 on serine 10 is required for its binding to CRM1 and nuclear export." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 14355-8, 2002 (PubMed).
Massenet, Pellizzoni, Paushkin et al.: "The SMN complex is associated with snRNPs throughout their cytoplasmic assembly pathway." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6533-41, 2002 (PubMed).
Kamura, Hara, Matsumoto et al.: "Cytoplasmic ubiquitin ligase KPC regulates proteolysis of p27(Kip1) at G1 phase." in: Nature cell biology, Vol. 6, Issue 12, pp. 1229-35, 2004 (PubMed).
|Reactivities||Human (32), Mouse (Murine) (23), Rat (Rattus) (23), Pig (Porcine) (18), Cow (Bovine) (6), Dog (Canine) (3), Xenopus laevis (2), Cat (Feline) (1), Chicken (1)|
|Applications||Western Blotting (WB) (22), Immunofluorescence (IF) (16), ELISA (13), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (6), AA 390-408 (2), Internal Region (1)|