Cullin 3 antibody (AA 565-684)

Catalog No. ABIN968679

This anti-Cullin 3 antibody is a Mouse Monoclonal antibody detecting Cullin 3 in WB and IF. Suitable for Human, Mouse, Rat, Chicken and Dog. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Cullin 3 antibody (AA 565-684) (ABIN968679)

Target: Cullin 3 (CUL3)

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cullin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 3-CUL

Product Details anti-Cullin 3 Antibody

Binding Specificity: AA 565-684

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CUL-3 aa. 565-684

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Cullin 3 antibody (ABIN968679)

Hori, Osaka, Chiba, Miyamoto, Okabayashi, Shimbara, Kato, Tanaka: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, (2000) (PubMed).

Singer, Gurian-West, Clurman, Roberts: "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells." in: Genes & development, Vol. 13, Issue 18, pp. 2375-87, (1999) (PubMed).

Target Details for Cullin 3

Target: Cullin 3 (CUL3)

Alternative Name: CUL-3

Background: The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul proteins, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. The human Cul family of proteins, Cul-1, -2, -3, -4A, -4B, and -5, have also been implicated in oncogenic processes. Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome. Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas. Cul-3 is widely expressed in normal cells and tissues, and shows increased expression in colon cancer cells. Cul-3 can associate with cyclin E in mammalian cells, and overexpression of Cul-3 targets cyclin E for ubiquination, while deletion of Cul-3 in mice leads to increased cyclin E accumulation and abnormal regulation of S-phase. Thus, the Cul family of proteins may have roles in cell cycle regulation and tumoriogenesis. This antibody is routinely tested by western blot analysis.

Molecular Weight: 89 kDa

Pathways: M Phase