COMT antibody (AA 26-141)

Catalog No. ABIN968704

This Mouse Monoclonal antibody specifically detects COMT in WB, IF and BI. It exhibits reactivity toward Rat and Mouse and has been mentioned in 3+ publications.

Quick Overview for COMT antibody (AA 26-141) (ABIN968704)

Target: COMT (Catechol-O-Methyltransferase (COMT))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This COMT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Clone: 4-COMT

Product Details anti-COMT Antibody

Binding Specificity: AA 26-141

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse COMT aa. 26-141

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-COMT antibody (ABIN968704)

Werner, Di Rocco, Prikhojan, Rempel, Bottiglieri, Bressman, Yahr: "COMT-dependent protection of dopaminergic neurons by methionine, dimethionine and S-adenosylmethionine (SAM) against L-dopa toxicity in vitro." in: Brain research, Vol. 893, Issue 1-2, pp. 278-81, (2001) (PubMed).

Gogos, Morgan, Luine, Santha, Ogawa, Pfaff, Karayiorgou: "Catechol-O-methyltransferase-deficient mice exhibit sexually dimorphic changes in catecholamine levels and behavior." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 17, pp. 9991-6, (1998) (PubMed).

Tilgmann, Melen, Lundström, Jalanko, Julkunen, Kalkkinen, Ulmanen: "Expression of recombinant soluble and membrane-bound catechol O-methyltransferase in eukaryotic cells and identification of the respective enzymes in rat brain." in: European journal of biochemistry / FEBS, Vol. 207, Issue 2, pp. 813-21, (1992) (PubMed).

Target Details for COMT

Target: COMT (Catechol-O-Methyltransferase (COMT))

Alternative Name: COMT

Background: Catechol-O-methyltransferase (COMT) and monoamine oxidase (MAO) are the major mammalian enzymes involved in the degradation of the catecholamine neurotransmitters, dopamine, norepinephrine, and epinephrine. COMT is a Mg2+-dependent enzyme that catalyzes the transfer of methyl groups from S-adenosyl methionine to a hydroxyl group of a catecholic substrate. Two forms of COMT are found in rat brain, a 24 kDa soluble COMT (S-COMT) and a 28 kDa membrane-bound COMT (MB-COMT). COMT is widely expressed in brain, but its importance in catecholamine neurotransmitter degradation relative to MAO varies in different brain regions. In addition, COMT may function primarily in extraneuronal areas, such as in glial cells and postsynaptic neurons. COMT-deficient mice have sex- and region-specific alterations in dopamine levels in the brain, and display impaired emotional reactivity and aggressive behavior. Thus, COMT-mediated degradation of catecholamines in the brain may have important roles in maintaining normal catecholamine levels, as well as normal social behavior. This antibody is routinely tested by western blot analysis.
Synonyms: Catechol-O-Methyltransferase

Molecular Weight: 24/28 kDa

Pathways: Steroid Hormone Biosynthesis, SARS-CoV-2 Protein Interactome