three Prime Repair Exonuclease 1 (TREX1) (AA 82-179) antibody
|Synonyms||HEXO, THEX1, 3'HEXO, MGC35395, Thex1, MGC108797, RGD1308378, ERI1, Eri-1, MGC134299, thex1, MGC110635, zgc:110635|
Alternatives Western Blotting (WB)
|2 references available|
|Price||Product not available in this region.|
|Description||DNA replication, repair, and recombination requires the excision of nucleotides from the DNA 3' termini. Many 3' to 5' exonucleases have been identified which catalyze the excision of monophosphates from the 3' termini of DNA. TREX1 and TREX2 are 3' to 5' exonucleases that contain three conserved exonuclease active site motifs (EASM) that may produce exonuclease activity. TREX1 and TREX2 are most closely related to the proofreading exonucleases of the bacterial replicative DNA polymerases and the RNase T enzymes. Recombinant expression of TREX1 and TREX2 demonstrates that they have exonuclease activity when oligonucleotide is present. TREX1 shows the greatest exonuclease activity with partial duplex DNA, and no activity with single-stranded RNA or an RNA-DNA partial duplex. In addition, reconstitution of TREX1 with DNA polymerase beta and DNA ligase III-XRCC1 facilitates accurate rejoining of a 3' mismatched base residue at a single-strand break. Thus, TREX1 and TREX2 are 3' to 5' exonucleases that may be important for excision of nucleotides during DNA replication, repair, and recombination.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||32 kDa|
Related Products: ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Immunology, DNA/RNA|
|Restrictions||For Research Use only|
|Western blot analysis of TREX1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells, ATCC CRL-1443). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1: 10,000 dilution of the mouse anti-mouse TREX1 antibody.|
Mazur, Perrino: "Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3'-->5' exonucleases." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19655-60, 1999 (PubMed).
Höss, Robins, Naven et al.: "A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein." in: The EMBO journal, Vol. 18, Issue 13, pp. 3868-75, 1999 (PubMed).
|Reactivities||Human (3), Mouse (Murine) (2), Rat (Rattus) (2)|
|Applications||ELISA (3), Western Blotting (WB) (3), Immunohistochemistry (IHC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)|
|Epitopes||Internal Region (1), Middle Region (1)|