CCCTC-Binding Factor (Zinc Finger Protein) (CTCF) (AA 184-290) antibody
|Synonyms||AW108038, CTCF, im:7152666, xctcf, p62, IMP2, IMP-2, VICKZ2|
Alternatives Western Blotting (WB), Immunofluorescence (IF)
|4 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Description||The family of nucleic acid-binding C2H2 type zinc finger transcription factors is divided into two classes. One class consists of small proteins (Gli1, Krox-20, WT1, Egr-1, and Sp1) with conserved zinc finger clusters of 3 to 5 units, while the other class (ZNF91, ZNF74, ZFP37, CTCF) can contain more than 10 zinc finger clusters. CTCF is a ubiquitously expressed, highly conserved transcription factor that contains 10 C2H2- and 1 C2H-type zinc-finger motifs. CTCF binds to and represses transcription at the promoter-proximal regions of c-myc oncogenes, while CTCF can bind to and activate transcription at the promoter for amyloid beta. Another mechanism of CTCF-mediated repression may include binding to insulator regions between enhancers and promoters resulting in enhancer blocking. In addition, CTCF gene may be a candidate tumor suppressor gene, since it localizes to a narrow cancer-associated chromosome region, and has been shown to have tumor-specific rearrangements in breast cancer patients. Thus, CTCF transcriptional regulation may be important for cell cycle progression, differentiation, apoptosis, and tumorigenesis. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Molecular Weight||140 kDa|
Related Products: ABIN968537, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
Klenova, Nicolas, Paterson et al.: "CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7612-24, 1994 (PubMed).
Filippova, Fagerlie, Klenova et al.: "An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 2802-13, 1996 (PubMed).
Filippova, Lindblom, Meincke et al.: "A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers." in: Genes, chromosomes & cancer, Vol. 22, Issue 1, pp. 26-36, 1998 (PubMed).
Bell, West, Felsenfeld: "The protein CTCF is required for the enhancer blocking activity of vertebrate insulators." in: Cell, Vol. 98, Issue 3, pp. 387-96, 1999 (PubMed).
|Hosts||Rabbit (40), Mouse (7), Rat (2), Sheep (1)|
|Reactivities||Human (48), Mouse (Murine) (33), Rat (Rattus) (28), Dog (Canine) (23), Cow (Bovine) (22), Horse (Equine) (19), Pig (Porcine) (19), Monkey (5), Chicken (3), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (29), Immunofluorescence (IF) (19), ELISA (15), Immunohistochemistry (IHC) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Fluorescence Microscopy (FM) (2), Immunocytochemistry (ICC) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (4), AA 650-700 (3), N-Term (2), AA 1-154 (1), AA 150-200 (1)|