MAD2L2 antibody (AA 81-180)

Catalog No. ABIN968795

The Mouse Monoclonal anti-MAD2L2 antibody has been validated for WB and IF. It is suitable to detect MAD2L2 in samples from Human and Dog. There are 2+ publications available.

Quick Overview for MAD2L2 antibody (AA 81-180) (ABIN968795)

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD2L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 14-MAD2B-Rev7

Product Details anti-MAD2L2 Antibody

Binding Specificity: AA 81-180

Cross-Reactivity: Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAD2B aa. 81-180

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968586, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-MAD2L2 antibody (ABIN968795)

Chen, Fang: "MAD2B is an inhibitor of the anaphase-promoting complex." in: Genes & development, Vol. 15, Issue 14, pp. 1765-70, (2001) (PubMed).

Murakumo, Roth, Ishii, Rasio, Numata, Croce, Fishel: "A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2." in: The Journal of biological chemistry, Vol. 275, Issue 6, pp. 4391-7, (2000) (PubMed).

Target Details for MAD2L2

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Alternative Name: MAD2B/Rev7

Background: Progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins. These events are of utmost importance at the checkpoints, or transition points, of the cell cycle. MAD2 (Mitotic Arrest-Deficient) is the human homolog of a yeast and Xenopus protein that is essential for spindle assembly during mitosis. Binding of affinity purified polyclonal antibodies to the MAD2 protein has been reported to prevent mitosis of HeLa cells. Furthermore, MAD2 is localized at the kinetochore of condensed chromosomes during mitosis and cells defective in the mitotic checkpoint have reduced levels of MAD2. MAD2 inhibits anaphase-promoting complex (APC) ubiquitin ligase activity,which is critical for controlling transitions in mitosis. This inhibition occurs through binding and suppression of CDC20 activation of APC. MAD2B, also known as Rev7, is a UV revertible gene that has 25% identity with MAD2 and also acts as an inhibitor of APC. Interestingly, MAD2B/Rev7 can bind MAD2 and interacts with the DNA polymerase zeta subunit Rev3. Thus, MAD2B may interact with mitotic checkpoint proteins and DNA repair proteins to regulate mitotic progression. This antibody is routinely tested by western blot analysis.
Synonyms: Mitotic Arrest Deficient-2B, Rev7

Molecular Weight: 26 kDa