Phosphoserine/threonine antibody

Antigen: Phosphoserine/threonine

Clonality: Monoclonal (22A)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968874

Additional Information

Format: Liquid

Isotype: IgG1

Clone: 22A

Description: Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Comments:

Related Products: ABIN968533, ABIN968887, ABIN967389, ABIN968871

Application Details

Application Notes: For western blotting, a dilution of 1:2500 may be a useful starting concentration for titrations.

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Neurology, Metabolism, Amino Acids

Restrictions: For Research Use only

Images

Immunofluorescence staining of phosphoserine (clone 19, MN ABIN968871) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

Immunofluorescence staining of phosphoserine (clone 19, MN ABIN968871) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555) treated with 10 nM calyculin A and 500 nM okadaic acid.

Immunofluorescence staining of phosphoserine/threonine (clone 22A, MN ABIN968873) on A431 cells (Human epithelial carcinoma, ATCC CRL-1555).

Publications

Product: Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, 1998 (PubMed).

Yan, Packer, Gooley et al.: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, 1998 (PubMed).

Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, 2002 (PubMed).