SPTAN1 antibody (AA 252-371)

Catalog No. ABIN968880

This anti-SPTAN1 antibody is a Mouse Monoclonal antibody detecting SPTAN1 in WB and IF. Suitable for Human, Mouse, Rat, Chicken and Dog. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SPTAN1 antibody (AA 252-371) (ABIN968880)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTAN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 35-alpha

Product Details anti-SPTAN1 Antibody

Binding Specificity: AA 252-371

Cross-Reactivity: Dog (Canine), Chicken

No Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human alpha-Spectrin II aa. 252-371

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-SPTAN1 antibody (ABIN968880)

Nicolas, Fournier, Galand, Malbert-Colas, Bournier, Kroviarski, Bourgeois, Camonis, Dhermy, Grandchamp, Lecomte: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, (2002) (PubMed).

Hu, Watanabe, Bennett: "Characterization of human brain cDNA encoding the general isoform of beta-spectrin." in: The Journal of biological chemistry, Vol. 267, Issue 26, pp. 18715-22, (1992) (PubMed).

Moon, McMahon: "Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4427-33, (1990) (PubMed).

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: alpha-Spectrin II

Background: Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, alpha and beta, which intertwine to form heterodimers that can self associate into elongated tetramers. alpha-spectin I and beta-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of alpha-spectin I and II with beta-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats along with various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. alpha-spectrin II is a widely expressed non-erythroid alpha-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. beta-spectrin II is a widely expressed non-erythroid beta-spectrin that contains a C-terminal region that interacts with alpha-spectrins and a PH domain. alpha-spectrin II and beta-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure.

Molecular Weight: 250 kDa

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization