Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 708-830) antibody

Details for Product No. ABIN968892
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Antigen
Synonyms 1200011N24Rik, AI225888, AI847218, lilr3, mKIAA0567, MGM1, NPG, NTG, largeG, fk62d06, wu:fb77a10, wu:fk62d06, zgc:92092
Epitope
AA 708-830
(8), (8), (4), (3), (3), (2), (1), (1)
Reactivity
Human
(36), (25), (23), (13), (12), (12), (6), (1)
Host
Mouse
(30), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(27), (10), (10), (10), (4), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 150 µg
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Catalog No. ABIN968892
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Immunogen Human OPA1
Clone 18
Isotype IgG1
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name OPA1
Background Three major GTP-binding protein families include trimeric and low molecular weight G-proteins, as well as a family of large proteins homologous to dynamin. The dynamin family contains proteins with diverse structure and function, but highly homologous N-terminal GTPase domains. A subgroup of the dynamin G-protein-binding family includes the mitochondrial proteins Drp1/Dnm1, Mgm1, and OPA1. The latter protein is mutated in dominant optic atrophy, a disease that involves loss of visual acuity and atrophy of the optic nerve. OPA1 is expressed in heart, brain, liver, and kidney. The sequence of OPA1 includes an N-terminal region that contains a mitochondrial targeting domain and three GTP-binding motifs. The overexpression of OPA1 in Cos-7 cells shows co-localization with cytochrome c in mitochondria, and leads to alterations in mitochondrial morphology from a characteristic tubuluar shape to a vesicular pattern. Thus, OPA1 may have roles in mitochondrial biogenesis that are critical for normal cell function. This antibody is routinely tested by western blot analysis.
Molecular Weight 80-100 kDa
Research Area Neurology
Comment

Related Products: ABIN968586, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 708-830) antibody Immunofluorescence staining of COS-7 cells (African Green Monkey SV40 transformed kidney cells, ATCC CRL-1651).
anti-Optic Atrophy 1 (Autosomal Dominant) (OPA1) (AA 708-830) antibody (2) Western blot analysis of OPA1 on a K-562 cell lysate (Human bone marrow myelogenous leukemia, ATCC CCL-243). Lane 1: 1:500, lane 2: 1000, lane 3: 1: 2000 dilution of the mouse anti- OPA1 antibody.
Product cited in: Delettre, Lenaers, Griffoin et al.: "Nuclear gene OPA1, encoding a mitochondrial dynamin-related protein, is mutated in dominant optic atrophy." in: Nature genetics, Vol. 26, Issue 2, pp. 207-10, 2000 (PubMed).

Alexander, Votruba, Pesch et al.: "OPA1, encoding a dynamin-related GTPase, is mutated in autosomal dominant optic atrophy linked to chromosome 3q28." in: Nature genetics, Vol. 26, Issue 2, pp. 211-5, 2000 (PubMed).

Background publications Misaka, Miyashita, Kubo: "Primary structure of a dynamin-related mouse mitochondrial GTPase and its distribution in brain, subcellular localization, and effect on mitochondrial morphology." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15834-42, 2002 (PubMed).

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