anti-EGF antibody (Epidermal Growth Factor)

Details for Product anti-EGF Antibody No. ABIN969091
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Synonyms HOMG4, URG, AI790464, CEGF
(303), (112), (80), (13), (12), (8), (7), (3), (3), (1)
(291), (145), (40), (12), (7), (3)
Clonality (Clone)
Monoclonal ()
This EGF antibody is un-conjugated
(64), (26), (22), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6)
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(309), (304), (74), (67), (60), (34), (33), (33), (31), (14), (10), (10), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1)
Pubmed 3 references available
Quantity 100 μL
Shipping to United States ( )
Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of human EGF expressed in E. coli.
Clone 9D7F11
Isotype IgG1
Purification Ascites
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Molecular Weight 134 kDa
Gene ID 1950
HGNC 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer,Lung ...
Immunohistochemistry (IHC) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 2: Immunohistochemical analysis of paraffin-embedded human Uterus tissues usin...
Western Blotting (WB) image for anti-EGF antibody (Epidermal Growth Factor) (ABIN969091) Figure 3: Western blot analysis using EGF mouse mAb against EGF-hIgGFc transfected HE...
Product cited in: Yacoub, McKinstry, Hinman et al.: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003 (PubMed).

Soubeyran, Kowanetz, Szymkiewicz et al.: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002 (PubMed).

Background publications Gout, Dhand, Panayotou et al.: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993 (PubMed).

Catalog No. ABIN969091
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