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Epidermal Growth Factor (EGF) antibody

Antigen

Epidermal Growth Factor (EGF)
Synonyms EGF, CEGF, LOC100218747, URG, HOMG4, AI790464
Clonality Monoclonal (9D7F11)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
2 references available
Catalog no. ABIN969091
Quantity 0.1ml  (Variants)
Price 363.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name EGF
Gene ID 1950
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 9D7F11
Description Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Synonyms: URG, HOMG4, EGF
Molecular Weight 134kDa

Application Details
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Application Notes Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascites
Buffer Ascitic fluid containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Research Area Cancer, Growth Factors
Restrictions For Research Use only

Publications
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Product Soubeyran, Kowanetz, Szymkiewicz et al.: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002 (PubMed).

Yacoub, McKinstry, Hinman et al.: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Epidermal Growth Factor (EGF)", type "Antibodies"
Hosts Rabbit (158), Mouse (83), Goat (15), Rat (3), Sheep (3), Chicken (1)
Reactivities Human (132), Mouse (Murine) (81), Rat (Rattus) (64), Pig (Porcine) (4), Wild boar (Sus scrofa) (4), Cow (Bovine) (3), Dog (Canine) (3), Rabbit (1), Sponges (Suberites domuncula) (1)
Applications ELISA (146), Western Blotting (WB) (123), Immunofluorescence (IF) (97), Flow Cytometry (FACS) (46), Immunohistochemistry (IHC) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (18), Neutralization (Neut) (13), Immunocytochemistry (ICC) (8), Immunoprecipitation (IP) (7), Functional Studies (Func) (6), Immunoelectron Microscopy (IEM) (6), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (4), ELISA (Capture) (2), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Radioimmunoassay (RIA) (2), ELISA (Detection) (1)
Conjugates Biotin (24), HRP (10), FITC (8), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), Gold (6), PE (6), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), PE-Cy3 (3), PE-Cy5 (3), PE-Cy5.5 (3), PE-Cy7 (3), Un-conjugated (3)
Epitopes Center (2), Extracellular Domain (2), Internal Region (2), AA 1-330 (1), pTyr1068 (1), pTyr1173 (1)