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Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) antibody

Antigen

Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)
Synonyms NOS, INOS, NOS2A, HEP-NOS, iNOS, Nos-2, Nos2a, i-NOS, NOS-II, eNOS, Nos-3, ecNOS, 2310065A03Rik, iNos, cNOS, EC-NOS, NOSIII, NOS3, NOS2, nos2, ECNOS
Clonality Monoclonal (6H2)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN969097
Quantity 0.1ml  (Variants)
Price 363.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name eNOS
Gene ID 4846
Immunogen Purified recombinant fragment of human eNOS expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 6H2
Description Endothelial nitric-oxide synthase (eNOS), also known as NOS3, it is an important enzyme in the cardiovascular system. It is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm.
Synonyms: ECNOS, NOS3
Molecular Weight 133kDa

Application Details
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Application Notes Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascites
Buffer Ascitic fluid containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Research Area Cancer, Enzymes, Metabolism, Inflammation
Restrictions For Research Use only

Publications
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Product Dimmeler, Fleming, Fisslthaler et al.: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, 1999 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3)", type "Antibodies"
Hosts Rabbit (200), Mouse (13)
Reactivities Human (173), Rat (Rattus) (131), Mouse (Murine) (126), Dog (Canine) (77), Cow (Bovine) (69), Pig (Porcine) (60), Sheep (Ovine) (56), Guinea Pig (55), Horse (Equine) (1), Monkey (1)
Applications Western Blotting (WB) (117), ELISA (86), Immunofluorescence (IF) (86), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Immunohistochemistry (IHC) (25), Immunocytochemistry (ICC) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (14), Immunoprecipitation (IP) (10), Flow Cytometry (FACS) (5), Immunoelectron Microscopy (IEM) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1), Radioimmunoassay (RIA) (1)
Conjugates Biotin (7), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes C-Term (33), pSer1177 (31), pSer113 (19), pThr495 (19), N-Term (6), Internal Region (5), pSer1176 (5), Ser1177 (4), pThr494 (4), AA 1181-1194 (3), pSer615,Internal Region (3), pThr494,Internal Region (3), AA 1186-1203 (2), pSer3 (2), AA 106-118 (1), AA 1162-1192 (1), AA 1163-1191 (1), AA 1179-1194 (1), AA 1202-1205 (1), AA 2-11 (1), AA 597-611 (1), Center (1), Internal Region,AA 713-743 (1), N-Term,AA 54-84 (1), Ser116 (1), pSer114 (1), pSer116 (1), pSer1179 (1), pSer396 (1), pSer615 (1), pSer617 (1), pSer635 (1)