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GATA Binding Protein 4 (GATA4) antibody
|Synonyms||MGC126629, Gata-4, GATA-4, GATA4, gata4, gata-4, xgata4, xGATA-4, MGC81427, GATA transcription factor 4, Gata4|
Human, Mouse (Murine)
Alternatives ELISA, Western Blotting (WB)
|2 references available|
|Price||407.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Purified recombinant fragment of human GATA4 expressed in E. Coli.|
GATA binding protein 4, also known as GATA4, it is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Buffer||Ascitic fluid containing 0.03% sodium azide.|
|Preservative||0.03% sodium azide|
|Storage||Store at 4℃, for long term storage, store at -20℃.|
|Research Area||Hypertrophy, Lineage Markers, Transcription Factors|
|Restrictions||For Research Use only|
Belaguli, Sepulveda, Nigam et al.: "Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7550-8, 2000 (PubMed).
Rajagopal, Ma, Obler et al.: "Spectrum of heart disease associated with murine and human GATA4 mutation." in: Journal of molecular and cellular cardiology, Vol. 43, Issue 6, pp. 677-85, 2007 (PubMed).
|Hosts||Rabbit (66), Mouse (7), Rat (2), Goat (1)|
|Reactivities||Human (73), Mouse (Murine) (53), Rat (Rattus) (52), Dog (Canine) (37), Chicken (36), Cow (Bovine) (36), Pig (Porcine) (36), Cat (Feline) (1)|
|Applications||Western Blotting (WB) (43), Immunofluorescence (IF) (41), ELISA (27), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Immunocytochemistry (ICC) (4), Immunohistochemistry (IHC) (4), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1)|
|Conjugates||Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)|
|Epitopes||pSer262 (18), Internal Region (3), C-Term (2), pSer105 (2), AA 27-212 (1), AA 330-380 (1), N-Term (1)|