GATA Binding Protein 4 (GATA4) antibody

Antigen: GATA Binding Protein 4 (GATA4)

Synonyms: MGC126629, Gata-4, GATA-4, GATA4, gata4, gata-4, xgata4, xGATA-4, MGC81427, GATA transcription factor 4, Gata4

Clonality: Monoclonal (6H10)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN969169

Additional Information

Alternative name: GATA4

Gene ID: 2626

Immunogen: Purified recombinant fragment of human GATA4 expressed in E. Coli.

Format: Liquid

Isotype: IgG2b

Clone: 6H10

Description: GATA binding protein 4, also known as GATA4, it is a 46 kDa member of the GATA family of zinc-finger transcription factors. Members of this family is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. These factors recognize the GATA motif which is present in the promoters of many genes. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs. GATA4 is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.
Synonyms: MGC126629

Molecular Weight: 46kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Hypertrophy, Lineage Markers, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Belaguli, Sepulveda, Nigam et al.: "Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators." in: Molecular and cellular biology, Vol. 20, Issue 20, pp. 7550-8, 2000 (PubMed).

Rajagopal, Ma, Obler et al.: "Spectrum of heart disease associated with murine and human GATA4 mutation." in: Journal of molecular and cellular cardiology, Vol. 43, Issue 6, pp. 677-85, 2007 (PubMed).