Lysine (K)-Specific Demethylase 3A (KDM3A) antibody

Antigen: Lysine (K)-Specific Demethylase 3A (KDM3A)

Synonyms: tsga, jmjd1, jhdm2a, jhmd2a, jmjd1a, TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, DKFZp686A24246, DKFZp686P07111, TGSA, Tsga, Jmjd1, KDM2A, JHDM2a, Jmjd1a, mKIAA0742, 1700105C21Rik, C230043E16Rik, KDM3A

Clonality: Monoclonal (2E1)

Host: Mouse

Reactivity: Human

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN969229

Additional Information

Alternative name: KDM3A

Gene ID: 55818

Immunogen: Purified recombinant fragment of human KDM3A expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 2E1

Description: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and 劥-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.
Synonyms: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, DKFZp686A24246, DKFZp686P07111, KDM3A

Molecular Weight: 147kDa

Synonyms: tsga, jmjd1, jhdm2a, jhmd2a, jmjd1a, TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A, KIAA0742, DKFZp686A24246, DKFZp686P07111, TGSA, Tsga, Jmjd1, KDM2A, JHDM2a, Jmjd1a, mKIAA0742, 1700105C21Rik, C230043E16Rik, KDM3A

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4° C, for long term storage, store at -20° C.

Restrictions: For Research Use only

Publications

Product: Nagase, Ishikawa, Suyama et al.: "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 5, Issue 5, pp. 277-86, 1999 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).