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Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) (AA 400-500) antibody

Antigen

Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4)
Synonyms HGK, NIK, FLH21957, FLJ10410, FLJ20373, FLJ90111, KIAA0687
Binding Site
Alternatives

AA 400-500
Clonality Monoclonal (4H9E7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
3 references available
Catalog no. ABIN969274
Quantity 0.1ml
Price 363.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Alternative name MAP4K4
Gene ID 9448
Immunogen Purified recombinant fragment of MAP4K4 (AA 400-500) expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 4H9E7
Description MAP4K4: mitogen-activated protein kinase kinase kinase kinase 4. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Synonyms: HGK, NIK

Application Details
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Application Notes Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Ascites
Buffer Ascitic fluid containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Research Area Signaling
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Becker, Huynh-Do, Holland et al.: "Nck-interacting Ste20 kinase couples Eph receptors to c-Jun N-terminal kinase and integrin activation." in: Molecular and cellular biology, Vol. 20, Issue 5, pp. 1537-45, 2000 (PubMed).

Poinat, De Arcangelis, Sookhareea et al.: "A conserved interaction between beta1 integrin/PAT-3 and Nck-interacting kinase/MIG-15 that mediates commissural axon navigation in C. elegans." in: Current biology : CB, Vol. 12, Issue 8, pp. 622-31, 2002 (PubMed).

Bouzakri, Zierath: "MAP4K4 gene silencing in human skeletal muscle prevents tumor necrosis factor-alpha-induced insulin resistance." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7783-9, 2007 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4)", type "Antibodies"
Hosts Rabbit (101), Mouse (12)
Reactivities Human (110), Mouse (Murine) (78), Chicken (20), Cow (Bovine) (20), Pig (Porcine) (20), Rat (Rattus) (20), Dog (Canine) (19), Horse (Equine) (19), Xenopus laevis (1), Zebrafish (1)
Applications Immunofluorescence (IF) (65), Western Blotting (WB) (40), ELISA (39), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (18), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunoelectron Microscopy (IEM) (4), Dot Blot (Dot) (3), Immunoprecipitation (IP) (3), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4), Alexa Flour 488 (1)
Epitopes pSer631 (21), pSer801 (21), pSer629 (20), C-Term (5), AA 400-500 (3), AA 931-947 (2), Center (1), Internal Region (1), Internal Region,AA 505-534 (1), N-Term (1), pSer63 (1)