PC4 and SFRS1 Interacting Protein 1 (PSIP1) antibody

Antigen: PC4 and SFRS1 Interacting Protein 1 (PSIP1)

Synonyms: p52, p75, PAIP, DFS70, LEDGF, PSIP2, MGC74712, Dfs70, Psip2, Ledgfa, Ledgfb, AA408851, AU015605, PSIP1, Ledgf, LEDGF/p75, MGC109898, zgc:109898

Clonality: Monoclonal (6E4)

Host: Mouse

Reactivity: Human, Monkey, Rat (Rattus)

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN969370

Additional Information

Alternative name: PSIP1

Gene ID: 11168

Immunogen: Purified recombinant fragment of human PSIP1 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 6E4

Description: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Tissue specificity: Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
Synonyms: p52, p75, PAIP, DFS70, LEDGF, PSIP2, MGC74712, PSIP1

Molecular Weight: 75kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Shun, Botbol, Li et al.: "Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity." in: Journal of virology, Vol. 82, Issue 23, pp. 11555-67, 2008 (PubMed).