SIRT1 antibody

Catalog No. ABIN969396

This anti-SIRT1 antibody is a Mouse Monoclonal antibody detecting SIRT1 in WB, IHC, ELISA, ICC and FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for SIRT1 antibody (ABIN969396)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1F3

Product Details anti-SIRT1 Antibody

Purpose: SIRT1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SIRT1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SIRT1 antibody (ABIN969396)

Xie, Park, Kim, Hwang, Oh, Park, Shong, Lee, Choi: "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation." in: The Journal of biological chemistry, Vol. 284, Issue 42, pp. 28762-74, (2009) (PubMed).

Cha, Noh, Kwon, Kim, Park, Park, Lee, Chung, Kang, Lee, Moon, Jang: "Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 13, pp. 4453-9, (2009) (PubMed).

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: The Sir2 protein in yeast is known to function in transcriptional silencing processes through the deacetylation of histones H3 and H4. The more recently described human homologue of Sir2, known as SIRT1, has been found to associate with the tumor suppressor protein p53.SIRT1 binds and deacetylates p53 with specificity for its C-terminal Lys382 residue in response to the upregulation of promyelocytic leukemia protein (PML) nuclear bodies or oncogenic Ras. The deacetylation of p53 SIRT1 has been shown to negatively regulate p53-mediated transcription, preventing cellular senescence and apoptosis induced by DNA damage and stress.SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues, with high expression in heart, brain and skeletal muscle and low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

Molecular Weight: 120 kDa

Gene ID: 23411

UniProt: Q96EB6

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling