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Metadherin (MTDH) antibody

Details for Product No. ABIN969496, Supplier: Login to see
Antigen
  • MGC75963
  • 2610103J23Rik
  • 3D3
  • 3D3/Lyric
  • AEG-1
  • AV353288
  • D8Bwg1112e
  • Lyric
  • AEG1
  • LYRIC
  • LYRIC/3D3
Reactivity
Human
75
24
22
Host
Mouse
38
36
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
59
28
20
20
13
11
10
6
4
2
1
1
Supplier
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Immunogen Purified recombinant fragment of human Metadherin expressed in E. coli.
Clone 2F11C3
Isotype IgG1
Purification Purified by protein G affinity chromatography
Alternative Name Metadherin (MTDH Antibody Abstract)
Background Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Molecular Weight 64 kDa
Gene ID 92140
HGNC 92140
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 50 % glycerol and 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-Metadherin (MTDH) antibody (ABIN969496) Figure 1: Flow cytometric analysis of Hela cells using Metadherin mouse mAb (green) a...
Immunohistochemistry (IHC) image for anti-Metadherin (MTDH) antibody (ABIN969496) Figure 2: Immunohistochemical analysis of paraffin-embedded human Liver tissues using...
Western Blotting (WB) image for anti-Metadherin (MTDH) antibody (ABIN969496) Figure 3: Western blot analysis using Metadherin mouse mAb against K562 (1), SKBR-3 (...
Product cited in: Britt, Yang, Yang et al.: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, 2004 (PubMed).

Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, 2004 (PubMed).