T Antigen (AA 218-352) antibody

Details for Product No. ABIN969569
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Antigen
Epitope
AA 218-352
(2), (2), (1), (1)
Reactivity
Human
(5), (1)
Host
Mouse
(3), (2)
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB), Fluorescence Microscopy (FM)
(5), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN969569
Quantity 0.1 mg
Price
396.00 $   Plus shipping costs $45.00
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Immunogen Purified recombinant fragment of human T (AA 218-352) expressed in E. coli.
Clone 6C12
Isotype IgG1
Purification Purified
Background Synonyms:
TFT
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Molecular Weight 47.4 kDa
Gene ID 6862
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-T Antigen (AA 218-352) antibody Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng)
anti-T Antigen (AA 218-352) antibody (2) Figure 3: Flow cytometric analysis of A549 cells using T mouse mAb (green) and negative control (red) .
anti-T Antigen (AA 218-352) antibody (3) Figure 1: Western blot analysis using T mAb against human T recombinant protein. (Expected MW is 40.3 kDa)
anti-T Antigen (AA 218-352) antibody (4) Figure 2: Western blot analysis using T mAb against HEK293 (1) and T (AA: 218-352) -hIgGFc transfected HEK293 (2) cell lysate.
Product cited in: Tirabosco, Mangham, Rosenberg et al.: "Brachyury expression in extra-axial skeletal and soft tissue chordomas: a marker that distinguishes chordoma from mixed tumor/myoepithelioma/parachordoma in soft tissue." in: The American journal of surgical pathology, Vol. 32, Issue 4, pp. 572-80, 2008 (PubMed).

Hosts (3), (2)
Reactivities (5), (1)
Applications (5), (2), (1), (1), (1)
Epitopes (2), (2), (1), (1)
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