Glutamate-Ammonia Ligase (GLUL) antibody

Antigen: Glutamate-Ammonia Ligase (GLUL)

Synonyms: glul, MGC64262, GS, GLNS, PIG43, PIG59, Glns, GLUL, MGC128403, xgs, F15E12.14, F15E12_14, GLUTAMINE SYNTHETASE

Clonality: Polyclonal

Host: Goat

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN99838

Additional Information

Alternative name: Glutamine Synthetase

Immunogen: Glutamine Synthetase [Microbial]

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code ABIN101212 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Concentration: 90.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Research Area: Neurology, Alzheimer's Disease, Amino Acids, Enzymes, Metabolism

Restrictions: For Research Use only