ErbB2/Her2 antibody (pTyr1248)
Quick Overview for ErbB2/Her2 antibody (pTyr1248) (ABIN126792)
Target
See all ErbB2/Her2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- pTyr1248
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Specificity
- This antibody recognizes erbB2 phosphorylated at tyrosine 1248 at 185 kDa and might crossreact with EGFR.
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Characteristics
- Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
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Purification
- Size exclusion chromatography
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Immunogen
- Phosphopeptide conjugated to hemocyanin
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Isotype
- IgG1
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anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 23-372) antibody
ErbB2/Her2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1248) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr877) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1112) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 Reactivity: Human WB, IF, ELISA, IHC (p), PLA Host: Mouse Monoclonal 3B1 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Pig, Rabbit, Cow, Hamster, Avian, Cat, Chicken, Donkey, Goat, Guinea Pig, Sheep WB, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 Reactivity: Human IHC, IF, FACS Host: Mouse Monoclonal ERBB2-2452 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 Reactivity: Human IHC Host: Mouse Monoclonal ERBB2-3078 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 1205-1255) antibodyErbB2/Her2 Reactivity: Human, Mouse WB, IHC, FACS, IP, ICC Host: Rabbit Monoclonal BLR241L unconjugated Recombinant Antibody
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 Reactivity: Human WB, IF, ELISA, IHC (p) Host: Mouse Monoclonal 4E1 unconjugated
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Application Notes
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1 mL H2O (15 min, RT).
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Buffer
- 1 mLPBS / 0.09 % Na-azide / PEG and Sucrose
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C/-80 °C
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Storage Comment
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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Alternative Name
- CD340 / ERBB2 / HER2
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Background
- ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
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Gene ID
- 2064
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UniProt
- P04626
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Target
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