D1-Nt antibody
This Rabbit Polyclonal antibody specifically detects D1-Nt in WB. It exhibits reactivity toward Arabidopsis, Nicotiana tabacum and Nostoc (Cyanobacteria).
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Catalog No. ABIN165361
$474.38
Plus shipping costs $50.00
200 μL
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United States
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Quick Overview for D1-Nt antibody (ABIN165361)
Target
D1-Nt (PsbA)
Reactivity
Arabidopsis, Nicotiana tabacum, Nostoc (Cyanobacteria)
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Specificity
- Higher plants. According to sequence homology antisera may reacts in Synechocystis only with PsbA2/3 but not with PsbA1. For analysis of cyanobacterial D1 proteins the dilution 1: 1.000 is recommended.
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Immunogen
- synthetic peptide against PsbA N- terminus (a. a. 1 – 17) from Arabidopsis thaliana PsbA protein.
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Isotype
- IgG
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Application Notes
- Western blot (1 : 2.000 for ECL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C
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- D1-Nt (PsbA)
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Background
- D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).
Target
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