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ELK1 antibody (pSer389)

This Rabbit Polyclonal antibody specifically detects ELK1 in IHC (p). It exhibits reactivity toward Human.

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Catalog No. ABIN196679

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Quick Overview for ELK1 antibody (pSer389) (ABIN196679)

Target

See all ELK1 Antibodies

ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This ELK1 antibody is un-conjugated

Application

Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

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Binding Specificity
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pSer389

Specificity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Elk-1 (phospho-Ser389) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 389.

Purification

Immunoaffinity chromatography.

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 389 (P-R-SP-P-A).
anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody
ELK1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer389) antibody
ELK1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (C-Term) antibody
ELK1 Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) antibody
ELK1 Reactivity: Human IHC, ELISA Host: Mouse Monoclonal 3H6D12 unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (C-Term) antibody
ELK1 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody
ELK1 Reactivity: Human, Mouse WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 67-166) antibody
ELK1 Reactivity: Human ELISA, PLA Host: Mouse Monoclonal 2G6 unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody
ELK1 Reactivity: Human, Mouse, Rat, Dog WB Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 247-403) antibody
ELK1 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-ELK1, Member of ETS Oncogene Family (ELK1) (AA 90-250) antibody
ELK1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

Application Notes

Suitable for use in Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store the antibody (in aliquots) at -20 °C.
Target

ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name

ELK1

Background

The transcription factor Elk1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by Elk1. Elk1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 (Ser383) is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.Synonyms: ETS domain-containing protein Elk-1, Elk-1

Gene ID

2002

NCBI Accession

NP_001107595

UniProt

P19419

Pathways

MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling