Phosphotyrosine antibody (phosphorylated)
Quick Overview for Phosphotyrosine antibody (phosphorylated) (ABIN126875)
Target
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- phosphorylated
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Purification
- Size exclusion chromatography
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Components
- incl. positive Control
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Immunogen
- Synthetic phosphopeptide conjugated to KLH
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Isotype
- IgG1
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anti-Phosphotyrosine antibody
Reactivity: Various Species WB, FACS, ICC Host: Mouse Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyReactivity: Various Species WB, IHC (p) Host: Mouse Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC Host: Mouse Monoclonal G104 Atto 390
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Application Notes
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA: 0.05 μg/mLImmunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1 mL H2O (15 min, RT).
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Buffer
- 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C/-80 °C
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Storage Comment
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
Expiry Date
- 12 months
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- Phosphotyrosine
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Target Type
- Amino Acid
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Background
- Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.
Target
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