VEGFA antibody (Biotin)
Quick Overview for VEGFA antibody (Biotin) (ABIN116249)
Target
See all VEGFA AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Specificity
- This antibody reacts with Vascular Endothelial Growth Factor.
-
Purification
- Affinity chromatography.
-
Immunogen
- Highly pure (>98%) recombinant hVEGF (human VEGF)
-
-
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody
VEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Mouse WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Goat Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
-
-
Application Notes
-
ELISA: To detect hVEGF by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
-
Buffer
- PBS, pH 7.2 without preservatives
-
Preservative
- Without preservative
-
Handling Advice
- Avoid repeated freezing and thawing.
-
Storage
- 4 °C/-20 °C
-
Storage Comment
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
-
-
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
Alternative Name
- VEGF-A
-
Background
- Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
-
Gene ID
- 7422
-
NCBI Accession
- NP_001020537
-
UniProt
- P15692
-
Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
-
