VEGFA antibody (Biotin)
Quick Overview for VEGFA antibody (Biotin) (ABIN181546)
Target
See all VEGFA AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- This antibody detects VEGF
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Purification
- Affinity chromatography
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Immunogen
- Highly pure (> 98 %) recombinant human VEGF
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody
VEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Mouse WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibodyVEGFA Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 6G5B5 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC, FACS Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA Reactivity: Human, Rat, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Goat Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA Reactivity: Human WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated
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Application Notes
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ELISA: Direct: To detect hVEGF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant VEGF. Western Blot: To detect VEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
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Buffer
- PBS, pH 7.2
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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Expiry Date
- 6 months
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Alternative Name
- VEGF-A
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Background
- Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
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Gene ID
- 7422
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NCBI Accession
- NP_001020537
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UniProt
- P15692
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Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
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