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VEGFA antibody (Isoform 165)

Name: VEGFA antibody (Isoform 165)
SKU: ABIN115672
Availability: InStock

This Rabbit Polyclonal antibody specifically detects VEGFA in IP, EIA and Func. It exhibits reactivity toward Human.

Catalog No. ABIN115672

$498.00

Plus shipping costs $50.00

0.2 mg

Shipping to: United States

Delivery in 1 to 2 Business Days

Quick Overview for VEGFA antibody (Isoform 165) (ABIN115672)

Target

See all VEGFA Antibodies

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This VEGFA antibody is un-conjugated

Application

Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Functional Studies (Func)

Discover our top product VEGFA Primary Antibody

Binding Specificity
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AA 27-191, Isoform 165

Specificity

The antibody will detect Human VEGF-A.

Purification

Protein A Affinity Chromatography

Immunogen

Highly purified Recombinant Human VEGF165 [Ala27-Arg191] produced in Insect cells (Cat.-No DA3514X)
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibody
VEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibody
VEGFA Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibody
VEGFA Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C2 unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) antibody
VEGFA Reactivity: Mouse WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 207-371) antibody
VEGFA Reactivity: Human WB, ELISA, ICC Host: Mouse Monoclonal 6G5B5 unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibody
VEGFA Reactivity: Human, Rat, Mouse WB, IHC, FACS Host: Rabbit Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibody
VEGFA Reactivity: Human, Rat, Mouse WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibody
VEGFA Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3F7 unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) antibody
VEGFA Reactivity: Human WB, ELISA, Neut Host: Goat Polyclonal unconjugated

anti-Vascular Endothelial Growth Factor A (VEGFA) antibody
VEGFA Reactivity: Human WB, ELISA, Neut Host: Rabbit Polyclonal unconjugated

Application Notes

Neutralization: 1-2.5 μg/mL, to yield one-half maximal inhibition (ND50) of the biologicalactivity of human VEGF (50 ng/mL). ELISA: 0.5 μg/mL, allows the detection of 0.5-1.0 ng/well of recombinant human VEGF165 orother VEGF splice forms. Immunoprecipitation: 1-5 μg/mL lysate or reaction volume.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Reconstitution

Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer

PBS, pH 7.4 containing no preservatives

Preservative

Without preservative

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Target

VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name

VEGF-A

Background

Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID

7422

NCBI Accession

NP_001020537

UniProt

P15692

Pathways

RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling