ErbB2/Her2 antibody (pTyr1112)
Quick Overview for ErbB2/Her2 antibody (pTyr1112) (ABIN126789)
Target
See all ErbB2/Her2 AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- pTyr1112
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Specificity
- This antibody specifically recognizes erbB2 phosphorylated at Tyrosine 1112 at 185 kDa.
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Characteristics
- Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
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Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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Components
- incl. positive Control
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Immunogen
- Synthetic phosphopetide conjugated to KLH. Epitope: phospho-Tyr1112LQRpYSED
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Isotype
- IgG1
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anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 23-372) antibody
ErbB2/Her2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1248) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr877) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1112) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 Reactivity: Human WB, IF, ELISA, IHC (p), PLA Host: Mouse Monoclonal 3B1 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 Reactivity: Human, Mouse, Rat, Dog, Monkey, Horse, Pig, Rabbit, Cow, Hamster, Avian, Cat, Chicken, Donkey, Goat, Guinea Pig, Sheep WB, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 Reactivity: Human IHC, IF, FACS Host: Mouse Monoclonal ERBB2-2452 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 Reactivity: Human IHC Host: Mouse Monoclonal ERBB2-3078 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 1205-1255) antibodyErbB2/Her2 Reactivity: Human, Mouse WB, IHC, FACS, IP, ICC Host: Rabbit Monoclonal BLR241L unconjugated Recombinant Antibody
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 Reactivity: Human WB, IF, ELISA, IHC (p) Host: Mouse Monoclonal 4E1 unconjugated
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Application Notes
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Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from EGF-treated SKOV-3 cells as positive control (please see Protocolsbelow for details). Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Protocol
- POSITIVE CONTROL CELL LYSATE: SKOV-3 EGF treatedFormat: Lyophilized cell lysate from SKOV-3 cells. Serum starved cells were treated for 15min with EGF. Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as immunoprecipitation. Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.
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Restrictions
- For Research Use only
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Reconstitution
- Restore with 1 mL H2O (15 min, RT).
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Buffer
- 1 mLPBS containing 0.09 % Sodium Azide/PEG and Sucrose.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C/-80 °C
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Storage Comment
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. -
Expiry Date
- 12 months
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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Alternative Name
- CD340 / ERBB2 / HER2
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Background
- ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
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Gene ID
- 2064
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UniProt
- P04626
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
Target
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