For best experience we recommend to activate Javascript in your browser.

Mouse IgG1 Isotype Control

Name: Mouse IgG1 Isotype Control
Brand: antibodies-online
SKU: ABIN457406
Price: 351.26 USD
Availability: InStock

FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p), Func Monoclonal MOPC-21 IgG1 kappa unconjugated

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) purified antibody (low endotoxin, concentration in sample 9 μg/mL). (Mouse IgG1 Isotype Control)

FACS

1 image

(9 references)

Catalog No. ABIN457406

Datasheet as PDF

Quick Overview for Mouse IgG1 Isotype Control (ABIN457406)

Target

IgG1

Clonality

Monoclonal

Application

Flow Cytometry (FACS), Isotype Control (IsoC), ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Clone

MOPC-21

Isotype

IgG1 kappa

Host
197

40

28

6

4

2

1

1
Mouse

Purpose

Mouse IgG1 Isotype Control Purified Low Endotoxin

Specificity

This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.

No Cross-Reactivity

Human, Rat

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Endotoxin Level

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Mouse IgG1 isotype control (Biotin)
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa Biotin

Mouse IgG1 isotype control (FITC)
FACS, ELISA Monoclonal 15H6 IgG1 FITC

Mouse IgG1 isotype control (PE)
FACS, ELISA Monoclonal 15H6 IgG1 PE

Mouse IgG1 isotype control (APC)
FACS, ELISA Monoclonal 15H6 IgG1 APC

Mouse IgG1 isotype control (SPRD)
FACS, ELISA Monoclonal 15H6 IgG1 SPRD

Mouse IgG1 isotype control
FACS, IsoC, ELISA, WB, IP, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa unconjugated

Mouse IgG1 isotype control (Biotin)
FACS, ELISA Monoclonal 15H6 IgG1 Biotin

Mouse IgG1 isotype control (APC-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy7

Mouse IgG1 isotype control (PE-Cy7)
FACS, ELISA Monoclonal 15H6 IgG1 PE-Cy7

Mouse IgG1 isotype control (APC-Cy5.5)
FACS, ELISA Monoclonal 15H6 IgG1 APC-Cy5.5

Application Notes

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Piao, Na, Seo, Hong, Sohn, Kwon, Lee, Kim: "Effects of general anaesthesia with an inhalational anaesthetic agent on the expression of exosomes in rats." in: International journal of medical sciences, Vol. 19, Issue 9, pp. 1399-1407, (2022) (PubMed).

Ni, Zhou, Song, Chen, Wang, Du, Zhang: "ELAVL1a is an immunocompetent protein that protects zebrafish embryos from bacterial infection." in: Communications biology, Vol. 4, Issue 1, pp. 251, (2021) (PubMed).

Klaile, Müller, Schäfer, Clauder, Feer, Heyl, Stock, Klassert, Zipfel, Singer, Slevogt: "Binding of Candida albicans to Human CEACAM1 and CEACAM6 Modulates the Inflammatory Response of Intestinal Epithelial Cells." in: mBio, Vol. 8, Issue 2, (2017) (PubMed).

Marchiani, Tamburrino, Giuliano, Nosi, Sarli, Gandini, Piomboni, Belmonte, Forti, Baldi, Muratori: "Sumo1-ylation of human spermatozoa and its relationship with semen quality." in: International journal of andrology, Vol. 34, Issue 6 Pt 1, pp. 581-93, (2011) (PubMed).

Rebetz, Tian, Persson, Widegren, Salford, Englund, Gisselsson, Fan: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, (2008) (PubMed).

Smed-Sörensen, Moll, Cheng, Loré, Norlin, Perbeck, Moody, Spetz, Sandberg: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, (2008) (PubMed).

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Bryceson, March, Barber, Ljunggren, Long: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, (2005) (PubMed).
Target

IgG1

Alternative Name

IgG1 kappa

Target Type

Antibody