Cathepsin D ELISA Kit

Catalog No. ABIN1672768

Mouse Cathepsin D ELISA Kit Colorimetric assay for quantification of Mouse Cathepsin D.

Quick Overview for Cathepsin D ELISA Kit (ABIN1672768)

Target: Cathepsin D (CTSD)

Binding Specificity: AA 21-410

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Cathepsin D ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cathepsin D

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: I21-L410

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: I21-L410

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, I21-L410) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cathepsin D amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Cathepsin D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Cathepsin D standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.13, Standard deviation: 0.033, CV(%): 2.9
• Sample 2: n=16, Mean(ng/ml): 3.29, Standard deviation: 0.115, CV(%): 3.5
• Sample 3: n=16, Mean(ng/ml): 6.68, Standard deviation: 0.247, CV(%): 3.7,
• Sample 1: n=24, Mean(ng/ml): 1.19, Standard deviation: 0.043, CV(%): 3.6
• Sample 2: n=24, Mean(ng/ml): 3.34, Standard deviation: 0.134, CV(%): 4
• Sample 3: n=24, Mean(ng/ml): 6.73, Standard deviation: 0.283, CV(%): 4.2

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Cathepsin D

Target: Cathepsin D (CTSD)

Alternative Name: CTSD

Background:

Protein Function: Acid protease active in intracellular protein breakdown.

Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Synonyms: Cathepsin D,3.4.23.5,Ctsd,

Full Gene Name: Cathepsin D

Cellular Localisation: Lysosome. Melanosome . Secreted, extracellular space.

Gene ID: 13033

UniProt: P18242

Pathways: Peptide Hormone Metabolism