P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA Kit

Antigen: P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP)

Synonyms: CD62, GRMP, PSEL, CD62P, GMP140, LECAM3, PADGEM, FLJ45155, Grmp, MGC129336, MGC129337, P-selectin, PSELECT, MGC124632, SELP

Reactivity: Mouse (Murine)

Application: ELISA

Catalog no.: ABIN411352

Additional Information

Characteristics: For quantitative detection of mouse P-Selectin in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: P-Selectin

Description: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 5 pg/ml. Range: 62.5pg/ml-4000pg/ml

Application Details

Principle: This mouse P-Selectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse P-Selectin specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse P-Selectin amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant mouse P-Selectin standard: 20ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

Images

P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) ELISA Kit