Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) ELISA Kit

Antigen: Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ)

Synonyms: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, adiponectin, APN, Acdc, apM1, 30kDa, adipo, Acrp30, ADIPOQ, MGC138982, ADN, MGC84292

Reactivity: Mouse (Murine)

Application: ELISA

Catalog no.: ABIN411385

Additional Information

Characteristics: For quantitative detection of mouse Adiponectin in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: Adiponectin

Description: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 136 (111-247) amino acids with the molecular mass of 19-23KDa.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 10 pg/ml. Range: 0.312ng/ml-20ng/ml

Application Details

Principle: This mouse Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse Adiponectin specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Adiponectin amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant mouse Adiponectin standard: 20ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

Images

Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) ELISA Kit