ADIPOQ ELISA Kit

Catalog No. ABIN411384

The Human ADIPOQ ELISA Kit (ABIN411384) is a Colorimetric ELISA Kit designed to quantify Human ADIPOQ. This ELISA Kit has been cited in 12 publications.

Quick Overview for ADIPOQ ELISA Kit (ABIN411384)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Binding Specificity: AA 19-244

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

Product Details ADIPOQ ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Purpose: Sandwich Human Adiponectin ELISA Kit to quantitate Human ADIPOQ in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: E19-N244

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 60 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.9%, 7.8%, 4.7%
Inter-Assay Precision (CV%): 6.5%, 9%, 5.2%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for ADIPOQ ELISA Kit (ABIN411384)

Buturak, Değirmencioğlu, Bayrak, Kırış, Karakurt, Demir, Sürgit, Ertürk: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." in: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).

Altay, Seçil, Demir, Atik, Akıncı, Özdemir Kutbay, Keskin Temeloğlu, Yıldırım Şimşir, Özışık, Demir, Eren, Tuna, Aytaç, Onay, Akıncı: "Determining residual adipose tissue characteristics with MRI in patients with various subtypes of lipodystrophy." in: Diagnostic and interventional radiology (Ankara, Turkey), Vol. 23, Issue 6, pp. 428-434, (2018) (PubMed).

Küme, Acar, Tuhan, Çatlı, Anık, Gürsoy Çalan, Böber, Abacı: "The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity." in: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 1, pp. 31-38, (2017) (PubMed).

Koh, Park: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." in: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).

Mohammadi, Paknahad: "Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study." in: Clinical nutrition research, Vol. 6, Issue 4, pp. 267-276, (2017) (PubMed).

Naini, Vahdat, Hedaiati, Shahzeidi, Pezeshki, Nasri et al.: "The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical ..." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 21, pp. 1, (2016) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: ADIPOQ

Background:

Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Gene Full Name: adiponectin, C1Q and collagen domain containing

Gene ID: 9370

UniProt: Q15848