Chemokine (C-X-C Motif) Ligand 16 (CXCL16) ELISA Kit

Antigen: Chemokine (C-X-C Motif) Ligand 16 (CXCL16)

Synonyms: CXCL16, SR-PSOX, Zmynd15, AV290116, BB024863, 0910001K24Rik, SRPSOX, CXCLG16, MGC137250, BLC, BCA1, ANGIE, BCA-1, BLR1L, ANGIE2, SCYB13, CXCL14, MGC127163

Reactivity: Mouse (Murine)

Application: ELISA

Catalog no.: ABIN411397

Additional Information

Characteristics: For quantitative detection of mouse CXCL16 in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Description: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2.1 These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine.2 CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen.1 Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells.1 CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo.1, 3 Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13.4 The standard product used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa.

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 1pg/ml. Range: 15.6pg/ml-1000pg/ml

Application Details

Principle: This mouse CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CXCL16 specific-specific monoclonal antibodies (clone No. 142417) were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL16 amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant mouse CXCL16 standard: 10ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

Images

Chemokine (C-X-C Motif) Ligand 16 (CXCL16) ELISA Kit