Histone Deacetylase 1 (HDAC1) ELISA Kit

Antigen: Histone Deacetylase 1 (HDAC1)

Synonyms: HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, Hdac1, Gm1824, EG15181, Hdac1-ps, ENSMUSG00000061062, CG7471, dHDAC-1, dHDAC1, DHDAC1, DmelCG7471, dmHDA401, DmHDAC1, drpd3, dRpd3, dRPD3, DRpd3, E(var)3-64BC, HDAC, HDAC-1, hdac1, HDAC1, l(3)04556, l(3)64Cc, rpd3, Rpd3/HDAC, rpd[3], Su(var)3-26, Su(var)326, Su(var)328, hdac1b, MGC53583, Rpd3, GB14706, yaf1, MGC69271, MGC81850, gon-10, rpd3l1, hdac1-b, MGC69289, hdac-1, MGC101582, chunp6919, zgc:65818, wu:fb19h11, wu:fi06f03, zgc:101582, mp:zf637-2-001987, MGC127235, MommeD5, MGC102534, MGC118085, ARABIDOPSIS HISTONE DEACETYLASE 1, ATHD1, ATRPD3A, F20D10.250, F20D10_250, HDA1, HDA19, HISTONE DEACETYLASE, HISTONE DEACETYLASE 1, HISTONE DEACETYLASE 19, HISTONE DEACETYLASE19, RPD3A, DKFZp468K082

Reactivity: Rat (Rattus)

Application: ELISA

Certificates: ISO 9001:2008, ISO 13485:2003

Catalog no.: ABIN432608

Additional Information

Specificity: This assay has high sensitivity and excellent specificity for detection of rat HDAC1. No significant cross-reactivity or interference was observed.

Synonyms: HD1, RPD3, GON-10, RPD3L1, DKFZp686H12203, Hdac1, Gm1824, EG15181, Hdac1-ps, ENSMUSG00000061062, CG7471, dHDAC-1, dHDAC1, DHDAC1, DmelCG7471, dmHDA401, DmHDAC1, drpd3, dRpd3, dRPD3, DRpd3, E(var)3-64BC, HDAC, HDAC-1, hdac1, HDAC1, l(3)04556, l(3)64Cc, rpd3, Rpd3/HDAC, rpd[3], Su(var)3-26, Su(var)326, Su(var)328, hdac1b, MGC53583, Rpd3, GB14706, yaf1, MGC69271, MGC81850, gon-10, rpd3l1, hdac1-b, MGC69289, hdac-1, MGC101582, chunp6919, zgc:65818, wu:fb19h11, wu:fi06f03, zgc:101582, mp:zf637-2-001987, MGC127235, MommeD5, MGC102534, MGC118085, ARABIDOPSIS HISTONE DEACETYLASE 1, ATHD1, ATRPD3A, F20D10.250, F20D10_250, HDA1, HDA19, HISTONE DEACETYLASE, HISTONE DEACETYLASE 1, HISTONE DEACETYLASE 19, HISTONE DEACETYLASE19, RPD3A, DKFZp468K082

Application Details

Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HDAC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HDAC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HDAC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of HDAC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: DNA/RNA, Transcription Factors, Enzymes

Restrictions: For Research Use only