anti-PAR1 Antibodies (IgG) ELISA Kit

Antigen: Anti-PAR1 Antibodies

Catalog no.: ABIN454033

Additional Information

Alternative name: anti-PAR1 Antibodies (IgG)

Description: The Thrombin-Receptor (PAR-1, F2R) is a seven transmembrane, G-protein- coupled receptor which is lokated in thrombocytes and endothelial cells. These enzymes cleave the N-terminus of the receptor, which in turn acts as a tethered ligand. In the cleaved state, part of the receptor itself acts as the agonist, causing a physiological response.. The occurrence of auto- antibodies against thrombin receptor is associated with the existence of different vascular diseases. The anti-Thrombin-Receptor -Antibody-EIA is designed for the determination of Antibodies (IgG) against the Thrombin-Receptor in serum and plasma.

Sensitivity: 5 standards between 2,5 U/ml and 40 U/mlcut off:20 U/ml Sample materials:Serum, Plasma Intraassay-Precision:7,83% Interassay-Precision:9,12%

Application Details

Principle: The anti-Thrombin-Receptor-Antibody-EIA is an antibody screening test. The Thrombin-Receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-Thrombin-Receptor-Antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Thrombin-Receptor-Antibody.

Protocol: Incubation of samples/ standards/ controls 100 µl 120 min, 4°C. Wash Incubation of detection antibody 100 µl 1 hr, room temperature. Wash Substrate incubation 100 µl/well 20 min, room temperature. Add Stopp solution 100 µl/well. Read at 450 nm

Restrictions: For Research Use only