COL1A1 ELISA Kit

Catalog No. ABIN454769

This Colorimetric ELISA kit is designed for the quantitative measurement of Human COL1A1.

Quick Overview for COL1A1 ELISA Kit (ABIN454769)

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.469-30 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Product Details COL1A1 ELISA Kit

Minimum Detection Limit: 0.469 ng/mL

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human Col ? concentrations in serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human Col ?.

Cross-Reactivity (Details): No significant cross-reactivity or interference was observed.

Sensitivity: < 0.117 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Characteristics: Homo sapiens,Human,Collagen alpha-1(I) chain,Alpha-1 type I collagen,COL1A1

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col Ⅰ. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Col Ⅰ. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Col Ⅰ, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Col Ⅰ in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: COL1A1

Background: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding