COL1A1 ELISA Kit

Catalog No. ABIN454771

Mouse COL1A1 ELISA Kit, Colorimetric assay for quantification of Mouse COL1A1.

Quick Overview for COL1A1 ELISA Kit (ABIN454771)

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma

Product Details COL1A1 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Purpose: This immunoassay kit allows for the specific measurement of mouse Collagen Type Ⅰ concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural mouse Collagen Type Ⅰ.

Characteristics: Mus musculus,Mouse,Collagen alpha-1(I) chain,Alpha-1 type I collagen,Col1a1,Cola1

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Collagen Type Ⅰ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Collagen Type Ⅰ present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Collagen Type Ⅰ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Collagen Type Ⅰ bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for COL1A1

Target: COL1A1 (Collagen, Type I, alpha 1 (COL1A1))

Alternative Name: Col1a1

Background: Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600Å in length and 15Å in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Type 1 collagen is the most abundant protein in the connective tissues, especially in tendon, skin and bone.

Pathways: Sensory Perception of Sound, Autophagy, Growth Factor Binding