IL23A ELISA Kit

Catalog No. ABIN921111

The Mouse IL23A ELISA Kit (ABIN921111) is a Colorimetric ELISA Kit designed to quantify Mouse IL23A. This ELISA Kit has been cited in 1 publication.

Quick Overview for IL23A ELISA Kit (ABIN921111)

Target: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Binding Specificity: AA 20-196, AA 23-335

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details IL23A ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Purpose: Sandwich Mouse IL-23 ELISA Kit to quantitate Mouse Il23a in cell culture supernatants, serum and plasma (heparin).

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: (M23-S335) + (L20-A196)

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 4.4%, 5%, 4.4%
Inter-Assay Precision (CV%): 6.2%, 6.9%, 5.1%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for IL23A ELISA Kit (ABIN921111)

Zhang, Ding, Zhao, Yang, Zhang, Zhang, Cui: "Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats." in: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1821-8, (2009) (PubMed).

Target Details for IL23A

Target: IL23A (Interleukin 23, alpha subunit p19 (IL23A))

Alternative Name: IL23A

Background:

Background: Interleukin-23 subunit alpha (IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19 (the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12 (stSG47812).

Gene Full Name: interleukin 23, alpha subunit p19

Gene ID: 83430

UniProt: Q9EQ14

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation