Caspase 1 Protein (CASP1) (AA 120-269, partial) (GST tag)

Catalog No. ABIN1046768

Recombinant Caspase 1 protein expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for Caspase 1 Protein (CASP1) (AA 120-269, partial) (GST tag) (ABIN1046768)

Target: Caspase 1 (CASP1)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 120-269, partial

Purification tag / Conjugate: This Caspase 1 protein is labelled with GST tag.

Sequence: NPAMPTSSGS EGNVKLCSLE EAQRIWKQKS AEIYPIMDKS SRTRLALIIC NEEFDSIPRR TGAEVDITGM TMLLQNLGYS VDVKKNLTAS DMTTELEAFA HRPEHKTSDS TFLVFMSHGI REGICGKKHS EQVPDILQLN AIFNMLNTKN

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Miller, Ayala, Egger, Raju, Yamin, Ding, Gaffney, Howard, Palyha, Rolando: "Purification and characterization of active human interleukin-1 beta-converting enzyme from THP.1 monocytic cells." in: The Journal of biological chemistry, Vol. 268, Issue 24, pp. 18062-9, (1993) (PubMed).

Thornberry, Bull, Calaycay, Chapman, Howard, Kostura, Miller, Molineaux, Weidner, Aunins: "A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes." in: Nature, Vol. 356, Issue 6372, pp. 768-74, (1992) (PubMed).

Cerretti, Kozlosky, Mosley, Nelson, Van Ness, Greenstreet, March, Kronheim, Druck, Cannizzaro: "Molecular cloning of the interleukin-1 beta converting enzyme." in: Science (New York, N.Y.), Vol. 256, Issue 5053, pp. 97-100, (1992) (PubMed).

Target Details

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1 protein

Background: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.

Molecular Weight: 44.2 kD

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome