Goat IgG Isotype Control

Catalog No. ABIN457738

Quick Overview for Goat IgG Isotype Control (ABIN457738)

Target: IgG

Application: Isotype Control (IsoC), ELISA, Western Blotting (WB), Dot Blot (DB), Immunodiffusion (ID), Binding Studies (Bind), Haemagglutination (H)

Isotype: IgG

Product Details

Host: Goat

Characteristics: Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to goat total serum proteins, to goat IgG, Fc specific and to goat IgG heavy and light chains.

Purification: The protein is isolated from plasma using ion exchange chromatography, affinity chromatography and gelfiltration. Chromatographically purified goat IgG

Purity: > = 98 % as determined by agar-gel electrophoresis. Tested in a concentration of 50 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains traces of transferrin.

Application Details

Application Notes: Purified goat IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec-trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.

Comment:

Lyophilized, stable white powder.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After opening the vial, the lyophilized conten t is reconstituted by adding 2 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.

Preservative: Without preservative

Handling Advice: Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.

Target Details

Target: IgG

Target Type: Antibody