Pig IgG Isotype Control

Catalog No. ABIN458573

Quick Overview for Pig IgG Isotype Control (ABIN458573)

Target: IgG

Application: Isotype Control (IsoC), ELISA, Western Blotting (WB), Dot Blot (DB), Immunoelectrophoresis (IEP), Immunodiffusion (ID), Binding Studies (Bind), Haemagglutination (H)

Isotype: IgG

Product Details

Host: Pig

Characteristics: Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to swine total serum proteins.

Purification: Chromatographically purified swine IgG

Purity: > = 98 % as determined by agar-gel electrophoresis. Tested in a concentration of 70 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains traces of IgA, transferrin and albumin.

Application Details

Application Notes: Purified swine IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.

Comment:

Lyophilized, stable white powder.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After opening the vial, the lyophilized content is reconstituted by adding 2 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.

Preservative: Without preservative

Handling Advice: Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.

Storage: 4 °C/-20 °C

Storage Comment: This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.

Target Details

Target: IgG

Target Type: Antibody