KAP1 Protein (AA 22-291, partial) (GST tag)

Catalog No. ABIN7479619

Recombinant KAP1 protein expressed in Escherichia coli (E. coli).

Quick Overview for KAP1 Protein (AA 22-291, partial) (GST tag) (ABIN7479619)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Particle-Enhanced Turbidimetric Assay (PETIA)

Purity: 90 %

Product Details

Protein Characteristics: AA 22-291, partial

Purification tag / Conjugate: This KAP1 protein is labelled with GST tag.

Sequence: PGEGSAGGEK RSTAPSAAAS ASASAAASSP AGGGAEALEL LEHCGVCRER LRPEREPRLL PCLHSACSAC LGPAAPAAAN SSGDGGAAGD GTVVDCPVCK QQCFSKDIVE NYFMRDSGSK AATDAQDANQ CCTSCEDNAP ATSYCVECSE PLCETCVEAH QRVKYTKDHT VRSTGPAKSR DGERTVYCNV HKHEPLVLFC ESCDTLTCRD CQLNAHKDHQ YQFLEDAVRN QRKLLASLVK RLGDKHATLQ KSTKEVRSSI RQVSDVQKRV

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS buffer, 20mM GSH

Storage: 4 °C

Target Details

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: Transcription intermediary factor 1-beta

Background: Background: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Synonyms: E3 SUMO-protein ligase TRIM28 EC=6.3.2.- KRAB-associated protein 1 Short name=KAP-1 KRAB-interacting protein 1 Short name=KRIP-1 Nuclear corepressor KAP-1 RING finger protein 96 Tripartite motif-containing protein 28.

Molecular Weight: 56.1 kD

NCBI Accession: NM_005762

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus