KAP1 Protein (AA 1-835) (GST tag)
(2 references)-
- Target See all KAP1 (TRIM28) Proteins
- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-835
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This KAP1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- TRIM28 (Human) Recombinant Protein (P01)
- Sequence
- MAASAAAASA AAASAASGSP GPGEGSAGGE KRSTAPSAAA SASASAAASS PAGGGAEALE LLEHCGVCRE RLRPEREPRL LPCLHSACSA CLGPAAPAAA NSSGDGGAAG DGTVVDCPVC KQQCFSKDIV ENYFMRDSGS KAATDAQDAN QCCTSCEDNA PATSYCVECS EPLCETCVEA HQRVKYTKDH TVRSTGPAKS RDGERTVYCN VHKHEPLVLF CESCDTLTCR DCQLNAHKDH QYQFLEDAVR NQRKLLASLV KRLGDKHATL QKSTKEVRSS IRQVSDVQKR VQVDVKMAIL QIMKELNKRG RVLVNDAQKV TEGQQERLER QHWTMTKIQK HQEHILRFAS WALESDNNTA LLLSKKLIYF QLHRALKMIV DPVEPHGEMK FQWDLNAWTK SAEAFGKIVA ERPGTNSTGP APMAPPRAPG PLSKQGSGSS QPMEVQEGYG FGSGDDPYSS AEPHVSGVKR SRSGEGEVSG LMRKVPRVSL ERLDLDLTAD SQPPVFKVFP GSTTEDYNLI VIERGAAAAA TGQPGTAPAG TPGAPPLAGM AIVKEEETEA AIGAPPTATE GPETKPVLMA LAEGPGAEGP RLASPSGSTS SGLEVVAPEG TSAPGGGPGT LDDSATICRV CQKPGDLVMC NQCEFCFHLD CHLPALQDVP GEEWSCSLCH VLPDLKEEDG SLSLDGADST GVVAKLSPAN QRKCERVLLA LFCHEPCRPL HQLATDSTFS LDQPGGTLDL TLIRARLQEK LSPPYSSPQE FAQDVGRMFK QFNKLTEDKA DVQSIIGLQR FFETRMNEAF GDTKFSAVLV EPPPMSLPGA GLSSQELSGG PGDGP
- Characteristics
- Human TRIM28 full-length ORF ( NP_005753.1, 1 a.a. - 835 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Tripartite Motif Containing 28 (TRIM28) (AA 379-524) protein (GST tag)
TRIM28 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Tripartite Motif Containing 28 (TRIM28) (AA 2-835) protein (His tag)Crystallography grade TRIM28 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tripartite Motif Containing 28 (TRIM28) (AA 2-834) protein (His tag)Crystallography grade TRIM28 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tripartite Motif Containing 28 (TRIM28) (AA 22-291), (partial) protein (GST tag)TRIM28 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, WB, ELISA, PETIA
Tripartite Motif Containing 28 (TRIM28) (AA 624-811) protein (His tag,DYKDDDDK Tag)TRIM28 Origin: Human Host: Escherichia coli (E. coli) Recombinant Bind, ScA
Tripartite Motif Containing 28 (TRIM28) (AA 366-802) protein (His tag)TRIM28 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Tripartite Motif Containing 28 (TRIM28) protein (Myc-DYKDDDDK Tag)TRIM28 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Myositis-specific anti-155/140 autoantibodies target transcription intermediary factor 1 family proteins. ..." in: Arthritis and rheumatism, Vol. 64, Issue 2, pp. 513-22, (2012) (PubMed).
: "Autoantibodies to transcription intermediary factor TIF1? associated with dermatomyositis." in: Arthritis research & therapy, Vol. 14, Issue 2, pp. R79, (2012) (PubMed).
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: "Myositis-specific anti-155/140 autoantibodies target transcription intermediary factor 1 family proteins. ..." in: Arthritis and rheumatism, Vol. 64, Issue 2, pp. 513-22, (2012) (PubMed).
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- Target
- KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
- Alternative Name
- TRIM28 (TRIM28 Products)
- Synonyms
- KAP1 Protein, RNF96 Protein, TF1B Protein, TIF1B Protein, TRIM28 Protein, kap1 Protein, rnf96 Protein, tf1b Protein, tif1b Protein, AA408787 Protein, KAP-1 Protein, KRIP-1 Protein, Tif1b Protein, Tif1beta Protein, Kap1 Protein, Krip1 Protein, TIF1BETA Protein, tripartite motif containing 28 Protein, tripartite motif containing 28 L homeolog Protein, tripartite motif-containing 28 Protein, TRIM28 Protein, trim28.L Protein, Trim28 Protein
- Background
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Full Gene Name: tripartite motif-containing 28
Synonyms: FLJ29029,KAP1,RNF96,TF1B,TIF1B - Gene ID
- 10155
- NCBI Accession
- NM_005762
- Pathways
- Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
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