OAS1 Protein (AA 1-364) (His tag)
Quick Overview for OAS1 Protein (AA 1-364) (His tag) (ABIN666789)
Target
See all OAS1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-364
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Purification tag / Conjugate
- This OAS1 protein is labelled with His tag.
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Sequence
- MMDLRNTPAK SLDKFIEDYL LPDTCFRMQI NHAIDIICGF LKERCFRGSS YPVCVSKVVK GGSSGKGTTL RGRSDADLVV FLSPLTTFQD QLNRRGEFIQ EIRRQLEACQ RERAFSVKFE VQAPRWGNPR ALSFVLSSLQ LGEGVEFDVL PAFDALGQLT GSYKPNPQIY VKLIEECTDL QKEGEFSTCF TELQRDFLKQ RPTKLKSLIR LVKHWYQNCK KKLGKLPPQY ALELLTVYAW ERGSMKTHFN TAQGFRTVLE LVINYQQLCI YWTKYYDFKN PIIEKYLRRQ LTKPRPVILD PADPTGNLGG GDPKGWRQLA QEAEAWLNYP CFKNWDGSPV SSWILLVRPP ASSLPFIPAP LHEA
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Characteristics
- OAS1, 1-364 aa, Human, His-tagged, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1) (AA 1-400) protein (His tag)
custom-made OAS1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1) (AA 1-400) protein (GST tag)OAS1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1) (AA 1-364) protein (His tag)OAS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol and 1 mM DTT
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- OAS1 (2',5'-Oligoadenylate Synthetase 1, 40/46kDa (OAS1))
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Alternative Name
- OAS1
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Background
- OAS1 is an enzyme included in the 2', 5'-oligoadenylate synthase family. This enzyme is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2', 5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. OAS1 may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. Recombinant OAS1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 43.9 kDa (384aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_002525
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Pathways
- Hepatitis C
Target
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